Taking advantage of standard DNA fragments in the standard sample, a standard curve of migration time against DNA size (base pair number) is worked out. And, aligning the upper maker and lower marker in the unknown sample according to those in the standard sample, all the migration times of unknown sample are adjusted. Thus, all the DNA sizes in the unknown sample are worked out using their adjusted migration times against the standard curve.
Meanwhile, taking advantage of standard DNA fragments in the standard sample, the correlation factor of the peak size against the DNA concentration is worked out for each DNA fragment, and all the DNA concentrations (in molarity or mass concentration) in the unknown sample are worked out by using the upper marker or the lower marker as a internal standard.
Manually redefine the peak for the upper maker or the lower maker is allowable.
A gel-like image is shown directly over an electropherogram.